Carmen Martinez Bernal. It is FP of Chemistry by training. Has the accreditation of
laboratory animal experimenter (Felasa cat. B) of the Generalitat de Catalunya.

She has been an internship teacher in training courses for laboratory caregivers/experimenters (Felasa category AO and B) and in the Genetics of Laboratory Animals and Experimental Models course (Autonomous University of Barcelona). He has experience in euthanasia of laboratory animals, animal welfare evaluation protocols, handling and experimentation with the following species: Rat, Mouse, Dog, Cat, Guinea Pig, Chicken, Chicken, Ferret, Pig, Mini pig, Chicks and Rabbit.

He has carried out safety and surgery studies in the Cardiovascular area in the Pharmacodynamics department of several companies (CIDA SAL, Harlan, Envigo). Among other techniques, he has experience in: cannulation of veins, jugular and carotid, and placement of Millar sensors (from the carotid to the heart and in the femoral artery) to check blood pressure; implantation of telemetry and infusion pumps; jugular, carotid, gallbladder, and vena cava cannulations in rats and rabbits; meniscus surgery in guinea pigs; safety studies, jugular and carotid vein cannulations and trachetomy in cats; water maze analysis in rat and mouse models; induction of edema by carrageenan in the rat; pharmacological and toxicological kinetics in dogs, monkeys, rats, mice, mini pigs and pigs; electrocardiograms and readings thereof as well as blood pressure measurement in dogs, monkeys and rats; different routes of administration in all species: (intravenous, oral, subcutaneous, intradermal, subplantar, intraduodenal and intramuscular; blood extraction by different routes (jugular, intracardiac, retroorbicular sinus, submandibular, saphenous).

Establishment of a murine model of myasthenia by immunization with the purifying acetylcholine receptor from the electrical organ of Tetronarce californica. The techniques used include affinity chromatography to purify the receptor, immunization of the animals, administration of the animals i.v., monitoring of clinical signs and performance of functional tests (hand-grip, treadmill). At the end of the experiments, euthanasia and extraction of tissues of interest (skeletal muscle, spleen, diaphragm). Intracardiac and submandibular blood extraction. Laboratory techniques mastered: Freezing of samples, sectioning with cryostat, immunohistochemistry, maintenance of primary cultures and cell lines, preparation of culture media, preparation of immunocytochemistry, laboratory management and maintenance (order management, material autoclaving, equipment calibration and maintenance, reagent preparation.