{"id":3195,"date":"2025-04-26T13:51:03","date_gmt":"2025-04-26T11:51:03","guid":{"rendered":"https:\/\/www.neuromuscularbcn.org\/?page_id=3195"},"modified":"2025-04-30T07:51:17","modified_gmt":"2025-04-30T05:51:17","slug":"proves-que-realitzen-al-laboratori-de-malalties-neuromusculars","status":"publish","type":"page","link":"https:\/\/www.neuromuscularbcn.org\/ca\/serveis\/laboratori-assistencial\/proves-que-realitzen-al-laboratori-de-malalties-neuromusculars\/","title":{"rendered":"Proves que realitzen al laboratori de malalties neuromusculars"},"content":{"rendered":"
ANTICOSSOS A S\u00c8RUM<\/h6>\n
\n
Protocol per a estudi d\u2019anticossos<\/h5>\n

Enviament d\u2019almenys 3 ml de s\u00e8rum (centrifugat, sense c\u00e8l\u00b7lules). Si la mostra est\u00e0 a temperatura ambient i es preveu un per\u00edode curt de recepci\u00f3 pot enviar-se a temperatura ambient. Si la mostra est\u00e0 congelada o es preveu un temps major de recepci\u00f3 es recomana enviament refrigerat o en gel sec.<\/p>\n<\/div>\n

    \n
  • Anticossos anti-contactina 1 (CIDP): 60 \u20ac<\/li>\n
  • Anticossos anti-neurofascina 155 (CIDP): 60 \u20ac<\/li>\n
  • Anticossos anti-neurofascina 186 (CIDP): 60 \u20ac<\/li>\n
  • Anticossos anti-LRP4 (Miast\u00e8nia Gravis seronegativa): 60 \u20ac<\/li>\n
  • Anticossos anti-CASPR2 (Neuromioton\u00eda): 60 \u20ac<\/li>\n<\/ul>\n
    \n
    <\/div>\n<\/div>\n<\/div>
    ESTUDI DE DISFERLINA EN MON\u00d2CITS:<\/h6>\n
    \n
    Protocol per a estudi de disferlina en mon\u00f2cits<\/h5>\n

    Recollir 1-2 tubs CPT de 10ml per pacient.\u00a0Ha de recollir-se a la vegada un tub CPT d\u2019un control (que no sigui familiar del pacient)<\/strong>\u00a0per evitar falsos resultats deguts a problemes en el transport dels tubs. \u00c9s imprescindible centrifugar els tubs abans del seu transport. Aix\u00f2 permet enviar les mostres a temperatura ambient durant unes 48 hores.<\/p>\n

      \n
    1. Recollir la sang al tub\u00a0BD Vacutainer\u00ae CPT \u2122<\/strong>\u00a0Tube amb Sodium Citrate amb el m\u00e8tode standard per BD Vacutainer\u00ae<\/li>\n
    2. Despr\u00e9s d\u2019obtenir la mostra de sang invertir el tub diverses vegades i deixar el tub vertical a temperatura ambient fins al seu centrifugat. Les mostres han de ser centrifugades a les\u00a02 hores<\/strong>\u00a0despr\u00e9s de l\u2019extracci\u00f3 per obtenir resultats \u00f2ptims.<\/li>\n
    3. Centrifugar el tub amb la mostra de sang a temperatura ambient (18-25\u00baC) en un rotor horitzontal durant un m\u00ednim de\u00a020 minuts<\/strong>\u00a0a\u00a01500 \u2013 1800 RCF (g)<\/strong><\/li>\n
    4. Despr\u00e9s del centrifugat\u00a0invertir el tub<\/strong>\u00a0suaument abans d\u2019enviar.<\/li>\n<\/ol>\n

      \u00a0ATENCI\u00d3:<\/strong>\u00a0Una excessiva velocitat de centrifugat (per sobre de 2000 RCF (g)) pot causar trencament del tub.<\/p>\n

        \n
      • Separaci\u00f3 de mon\u00f2cits i estudi de disferlina: 395,75 \u20ac<\/li>\n<\/ul>\n<\/div>\n<\/div>
        ESTUDI DE BI\u00d2PSIA MUSCULAR<\/h6>\n
        \n
        Protocol per a EL estudi de BI\u00d2PSIA MUSCULAR<\/h5>\n

        Si la bi\u00f2psia muscular est\u00e0 feta en un hospital amb experi\u00e8ncia en congelaci\u00f3 de m\u00fascul (amb metilbut\u00e0 refredat en nitrogen l\u00edquid); aquesta s\u2019ha d\u2019enviar en abundant gel sec (10 kg) pr\u00e8viament congelada.<\/p>\n

        En el cas de ser un hospital sense experi\u00e8ncia en la congelaci\u00f3 de m\u00fascul, \u00e9s recomanable sol\u00b7licitar el trasllat del pacient per a la realitzaci\u00f3 de la bi\u00f2psia muscular directament al nostre hospital.<\/p>\n

        Les proves realitzades en el nostre laboratori s\u00f3n les seg\u00fcents:<\/p>\n<\/div>\n

          \n
        • Tinci\u00f3 histoqu\u00edmica de citocrom oxidasa (COX) \u2192 81,90 \u20ac<\/li>\n
        • Tinci\u00f3 histoqu\u00edmica de succinat deshidrogenasa (SDH) \u00e0 74,08 \u20ac<\/li>\n
        • Tinci\u00f3 histoqu\u00edmica de fosforilasa (FSA) \u00e0 52,63 \u20ac<\/li>\n
        • Tinci\u00f3 histoqu\u00edmica de fosfofructoquinasa (PFK) \u00e0 217,88 \u20ac<\/li>\n
        • Tinci\u00f3 histoqu\u00edmica de mioadenilato deaminasa (MADA) \u00e0 63,42 \u20ac<\/li>\n
        • Immunohistoqu\u00edmica de 4 anticossos (distr\u00f2fia muscular) \u2192 605.43 \u20ac<\/li>\n
        • Immunohistoqu\u00edmica de 8 anticossos (distr\u00f2fia muscular) A05 \u20ac<\/li>\n
        • Perfil miopaties inflamat\u00f2ries \u2192 648,69 \u20ac<\/li>\n
        • Western blot: 4 anticossos \u2192 605,43 \u20ac<\/li>\n
        • Western blot: 8 anticossos \u2192 1.124,34 \u20ac<\/li>\n<\/ul>\n<\/div>

          Per realitzar un correcte estudi de la bi\u00f2psia muscular, \u00e9s imprescindible la realitzaci\u00f3 de talls criost\u00e0tics i una bateria de proves en el servei d\u2019Anatomia Patol\u00f2gica<\/strong> del nostre hospital, que t\u00e9 un cost total de 294,84 \u20ac<\/strong>. Aquestes proves es realitzen autom\u00e0ticament en totes les bi\u00f2psies rebudes, i s\u00f3n les seg\u00fcents:<\/p>\n

            \n
          • Tinci\u00f3 Hematoxilina \u2013 Eosina<\/li>\n
          • Tinci\u00f3 tricr\u00f2mic de Gomori<\/li>\n
          • Tinci\u00f3 NADH<\/li>\n
          • Tinci\u00f3 ATPasa<\/li>\n
          • Tinci\u00f3 PAS<\/li>\n
          • Tinci\u00f3\u00a0Oil-Xarxa<\/li>\n<\/ul>\n
            \n
            <\/div>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":419,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"100-width.php","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"class_list":["post-3195","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"\nProves que realitzen al laboratori de malalties neuromusculars - Neuromuscular BCN<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neuromuscularbcn.org\/ca\/serveis\/laboratori-assistencial\/proves-que-realitzen-al-laboratori-de-malalties-neuromusculars\/\" \/>\n<meta property=\"og:locale\" content=\"ca_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Proves que realitzen al laboratori de malalties neuromusculars - 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